The Experimental Study Of SIN To The Mesangial Proliferative Glomerulonephritis In Rats

Objective: In this study, model of mesangial proliferative glomerulonephritis(MsPGN) was made with immunization. The MsPGN rats were cured by sinomenine(SIN). Proteinuria plasma protein, blood-fat, renal function, renal pathomorpholog- y, the expression of renal TNF-αand TNF-αmRNA were observed. The effect and mechanism of SIN on MsPGN were investigated so as to provid experimental data.Method : Thirty-six male Sprague-Dawley rats were randomly divided into four groups: Normal, Model, SIN and TWP group. 24h urine protein was determined with the trichloroacetic acid turbidimetry. SerumTP, Alb, TC ,TG , HDL , LDL,BUN,Scr were assayed with the automaticbiochemistry analyzer. Each group of the mesangiu area and the membrane cell (GMC) and the extracellular matrix (ECM) were observed with light microscope, electron microscope. The expression of TNF-αwas observed with the immunohistochemical staining and reverse transcription polymerase chain reaction (RT-PCR). The sectionof HE,Masson,PAS staining and immunohisto- chemistry staining was analyzed with the pathology image analysis system.Result:(1) 24h urinary protein quantitative analysis...