| Objective: To investigate the efiFects of mitogen activated protein kinase (MAPK) andNrf2(Nuclear factor-E2 related factor) on γ-glutamylcysteine synthase influenced by glucocorticosteroid and the inhibitor of protein tyrosine kinase genistein in lung of guinea pigs with bronchial asthma and the inflammatory cell in sputum with bronchial asthma.Methods : The whole experiment was divided into tow parts : animal research and clinicresearch.Part 1. Animal research: 20 adult male guinea pigs were randomly divided into control group (group A) ,asthmatic group (group B),dexamethasone group(group C) and genistein group (group D) (10 in each group). Asthmatic model was established by ovalbumin intraperitoneal combined with inhalation .The total cell and the proportion of inflammation cell in bronchoalveolar lavage fluid (BALF) were measured. The reduced glutathione(GSH) and total GSH in BALF and lung tissue were examined respectively ;Inflammatory cell infiltration and index of remodeling of bronchiole were measured; The γ-GCS- h mRNA in lung tissue was examined by in situ hybridization , Immunohistochemistry detected the expression of phosphorylated extracellular signal regulated kinase (p-ERK), phosphrylated c-Jun amino terminal kinase (p-JNK) , phosphorylated P38(p-P38), Nrf2 andγ-GCS in lung tissue, RT-PCR measured the expression of γ-GCS-h mRNA and Nrf2 mRNA in lung tissue, western blot detected the expression of p-ERK, p-JNK, p-P38 , total ERK, total JNK, total P38 and Nrf2 in lung tissue. The activity of γ-GCS was measured by coupled enzyme assay. Part 2. clinic research.Asthmatic patient were divided into two group according to the treatment.The patients who were treated by inhaling budesonide and salbutamol were due to group A and the others inhaling salbutamol only were due to group B;the health person acted as control group(group C) (11 cases in each group).The sputum were induced by inhaling 3% salt water.The total cell ,the proportion of inflammatory cell in sputum; the...
|
PDF Full Text Request