Influence Of Hyperglycemia On The Expressions Of NF-κB And Caspase-3 After Reperfusion In Experimental Brain Ischemia Rats

Background: Hyperglycemia can aggravates cerebral ischemic reperfusion injury. In the past the studies were mostly focused on the necrosis. Now it is found that apoptosis plays an important role in the brain injury after reperfusion. There are many studies on the roles of NF-κB and Caspase-3 in the cell apoptosis. But less studies are investigated about the influence of hyperglycemia on the expressions of NF-κB and Caspase-3 in reperfusion injury of the brain. It is not clear whether hyperglycemia can aggravate the apoptosis via NF-κB and Caspase-3.Objective: The project is to observe the infarct volume, expressions of NF-κB and Caspase-3 in the brain after ischemic reperfusion under the condition of hyperglycemia , investigate the mechanism of apoptosis induced by hyperglycemia, then provide some theoretical foundation for the therapy of cerebral ischemic disease under the condition of hyperglycemia.Methods: Thirty-six healthy male Sprague-Dawley(SD) rats were randomly divided into three groups: false operation group(n=4); ischemia control group(n=16, subdivided into four groups, 2h, 4h, 6h, 24h) and hyperglycemia group(n=16, subdivided into four groups, 2h, 4h, 6h, 24h). False operation group: sewing up the skin after exposing the common carotid artery(CCA) in the right side. Ischemia control group: the blood of vena caudalis was measured before NS was injected into the abdominal cavity(6ml/kg). After half an hour the blood of vena caudalis was measured again then the models were established according to Zea Longa: exposing the right common carotid artery, external carotid artery(ECA), internal...