The Impact Of Hypoxia On The Expression Of HIF-1α, VEGF In Hela Cell.

Background Recently,with the deep research of malignant neoplasm.some author discover that HIF-1αmaybe the key translator-factor to adapt the hapoxia condition for most cell. HIF-1αalso can indirectly modulate the expression of VEGFthat promote the development of the neoplasm.Normally,the neoplasm which develp fast has more stoma and vessel but lessconnective tissue,the neoplasm which develp slow is converse.Without new vessels,neoplasm whose diameter exceed 1-2mm or count to 107 would grow no more ,so inducing the new vessels is essential to the developmet ,infiltration, metastasis of the neoplasm.the over-development of the neoplasm will cause to hypoxia.then hypoxia stimulate more new vessels,this improve the development of neoplasm.Objective This assay is to investigate the impact of hypoxia on the expression of HIF-1αand VEGF in Hela cell by induing hypoxia with cobalt choride(250μmol/l). To study the correlation between HIF-1αand VEGF.This will help to the study the pathological mechanism of cervical cancer . may provide new method to the treament of cervical cancer through blocking up the expression of HIF-1αand VEGF.Methods1. Primary cultured Hela cell were trated with cobalt chloride .2. The expression of HIF-1α,VEGF were detected by using immunocytochemistry,the level of the expression of HIF-1α,VEGF were compared between non-stimulated and cobalt chloride-stimulated (2h,4h,6h,8h,12h,16h,24h) Hela cell .3. The expression of HIF-1α,VEGF were detected by using western blot ,the level of the expression of HIF-1α,VEGF were compared...