Construction Of Full-length HCV CDNA With Cell Culture-adaptive Mutations And Preparation Of Infectious RNA By In Vitro Transcription

Hepatitis C is one of the seriously infectious diseases which are transmitted through blood around the worldwid. According to the statistic data provided by WHO, there are about 170 million HCV carriers in the world. An important feature of HCV is that 80% of infected individuals develops chronic infections, which can lead to severe sequelae, such as liver cirrhosis and hepatocellular carcinoma. More than 2% of the global population is chronically infected with HCV. About 20 ~ 30% of HCV patiens would develop to cirrhosis. Consequently, hepatitis C has been viewed as a global severe social public health problem.Hepatitis C virus (HCV), the causal agent of Hepatitis C, is an enveloped positive-sense single-stranded RNA virus that has been classified in a separate genus (Hepacivirus) of the Flaviviridae. The HCV genome consists of 5' and...