Effects Of N-butanol Extrate Of Radix Morindae Officinallis On Anoxia/reoxygenafion Apoptosis In Caltured Neonatal Rat Cardiomyocytes

Apoptosis and necrosis are two different forms of cell death in myocardial ischemia/reperfusion injury (IRI). Apoptosis is a genetically determined, active process with typical morphological and biological changes, these changes are regulated by the actious of series of genes. So there is important significance to study apoptosis in myocardial is ischemia/reperfusion injury.The root of Morindae officinalis (RMO) is used to treat rheumatoid arthritis and impotence in the traditional medicine. The previous study has demonstrated that RMO has had eridently protective effects on myocardial ischemia/reperfusion injury induced by ligation of coronary atery and on the model of ischemia/reperfusion in cultured neonatal rat cardiomyocytes, but there are no reports so far about whether RMO can inhibit cardiomyocyte apoptosis. The puropse of this study is to investigate the effect of the n-butanol extracted from Radix Morinda officinalis (BEM) on Anoxia/ roxygenation apoptosis in cultured neonatal rat cardiomyocytes. MethodsThe hearts were isolated from 1~3 -day-old Wistar rats and the ventricles were minced in phosphate buffer solution. The myocardial cells were dissociated with...