Construction Of Recombinant Adenovirus Containing Human IFNβ Gene By Homogenous Recombination In E.coli.

Background and ObjectivesInterferons(IFNs) are well known for both their antiproliferative effect and immunomodulatory activity treating on tumor in vitro and in vivo, and they are the first cytokines applied clinically in human cancer. Type I IFNs, especially IFNβ , exhibit a more marked growth inhibitory activity against most human tumors. People all over the world are trying to explore the complicated mechanism of their direct and cooperative effect. Like many cytokines, IFN β has a very low blood concentration under physiological conditions because the body has a precisely modulation of its secretion to keep the balance. When the blood concentration of IFNs reach the maximum dose by given parenteral protein administration, complicated even dangerous systemic toxicities will appear instead of antiproliferative effect. It is hard to rely on IFN β 's concentration by this means because of it's rapid protein clearance and extremely short half-life in blood system. That is to say, systemic promotion of IFN β 's concentration is far beyond our limitation, and local IFN β gene therapy provides a promising strategy for the treatment of humans tumors.Compared with other gene therapy vectors, adenovirus vector is a mature...