Human Mesenchymal Stem Cells Inhibit Maturation And Function Of Monocyte-derived Dendritic Cells

Aim Mesenchymal stem cells discovered recently is a kind of adult stemcells derived from mesoblast .It has the commonness of stem cells that is capable of self-renewal and differentiating into many cell lineages.Recent studies show that MSC have potential application in transplantion due to its unique characteristics ,such as low immunogenicity,supporting hemopoiesis and immunoregulatory function which is the focus in transplantation field.But its immunoregulatory mechanism is not yet clear.Dendritic cells is the most powerful antigen-presentation cells in vivo which can activate naive T cells and plays an important role in immune response.DC can induce graft versus host disease or immune tolerance after hematopoietic stem cell transplantation, and the investigations on the mechanism of MSC's effect on DC may provide possible means to prevent or cure GVHD. The mechanism may involve DC subset,maturation and cytokine .The present study investigated the effects of MSC supernatant on DC maturation and cytokine,and it will provide data for its clinical application.Materials and methods1. MSC's isolation,cultivation and identificationMononuclear cells (MNC) were isolated from marrow aspirates drawn from healthy adults by Ficoll-Paque density gradient centrifugation. Cells were cultured in low glucose DMEM containing 15% febal calf serum(FCS). Fluorescein...