Extraction Matrine With AOT Reversed Micelle And The Anti-fungal Activity

The use of reversed micelle in extraction of biological molecules has the advantages of mild operational conditions, high extraction efficiency, low cost and solvent recycling. Matrine and Oxymatrine bearing many physiological functions such as anti-hepatitis B, anticancer and antivirus etc., becomes one of the sustaining hotspots of research, exploitation and utilization home and abroad.The process of matrine extraction with AOT-isooctane reversed micelle system has been systematically studied in this paper, and the effect on matrine extraction from the factors including pH, Na+, K+, Ca2+, W0 of AOT-isooctane reversed micelle, temperature and time were discussed. PH value is the main factor to effect extraction rate of matrine. While the pH value is during 7.0 and 12.0, Extraction rate of matrine rises with the increase of pH, but when pH>12, the rising speed will dramatically slow down and the purity of matrine will decrease. Low pH can increase the purity of matrine, but when pH<7, organic facies would emulsify in the process, which spoiled AOT-issooctane reversed micelle and stops the extraction. Species and concentration of ions can also effect the extraction. The optimum conditions have been obtained with pH=12, W0=30, T=35℃, ratio of organic to aqueous phase 1:1 and extraction time 30 min. Under these conditions, the extraction rate and purity of matrine are respectively 70.3% and 66.8%.Method of capillary electrophoresis with UV detection has been applied to the quantitative analysis of matrine, Ephedrini is taken as inner label. The optimum conditions have been received with 30 kV as separation voltage, 20 m mol/L barax-30 m mol/L SDS-35% V methanol as running buffer, electrophoretic injection 30 kV×4 sec, detection wavelength 208 nm. The quantitative analysis of matrine and Oxymatrine can be quickly achieved in these conditions.The influence of matrine as an anti-fungal drug was also studied. The MIC and MFC of matrine to Trichophyton rubrum are respectively 0.1 mg/mL and 10 mg/mL, and those to E. floccosum 0.2 mg/mL and 20 mg/mL; the MIC and MFC of raw matrine to Trichophyton rubrum are respectively 0.5 mg/mL and 50 mg/mL, and those to E. floccosum 1.0 mg/mL and 20 mg/mL. The cell wall and cell membrane of epiphyte are probably broken by matrine and fungal death is led to, as a result of the possible antifungal mechanism of matrine.