| Objective: In this study, the rat model of focal cerebral ischemia reperfusion was established with the middle cerebral artery occlusion by reversible inserting a nylon thread method ,to study the anti-cerebral ischemia reperfusion injury effects of TMZ and its mechanisms.Methods: The model of local ischemia-reperfusion in rats was established with the suture occluded method invented by Zea Longa and improved properly in our lab. Male SD rats were randomly divided into four groups: rats in group1 (sham)only received anesthesia and vessel separation; rats in group 2 received ischemia reperfusion operation and NS ig; in group 3 and 4 received ischemia-reperfusion and TMZ 5 mg/kg and 10mg/kg daily respectively. Rats in each group had been administered drugs for 2 weeks and then operation was done. Two hours later, we drew out the suture to recover perfusion. The neurological deficit score of rats in each group was evaluated after 24h ischemia-reperfusion. Then the subjects were killed .The infarct volume and water content were measured and pathological examination was performed. Influence of TMZ on activity of SOD, ATPase and the content of MDA in cerebral cortex were tested by spectrophotometry.Results: 1.All animals in each group manifested neuronal function disorder after 24h reperfusion following 2h ischemia. The symptom of model rats were especially marked. It was found that TMZ could improve the symptom of neurological deficit after cerebral ischemia reperfusion ,and decrease significantly the infarct volume and water content of cerebrum. There were great difference comparaed with the model group(P<0.05). Pathological findings showed that there were wide-ranging cerebral edema and neuronal cells necrosis. The finding also showed that TMZ treatment groups were able to abate pathological changes. 2. The activity of SOD and ATPase was remarkably lower and the content of MDA was higher in ischemia reperfusion group... |
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