The Role Of JNK In The Gene Expression In 3T3-L1 Adipocytes

Objective:Adipose tissue is known to be a strong endocrine organ with the ability to secret numerous adipokines, including adiponectin and resistin, which can influence insulin action and glucose transport, contributing to the pathogenesis of obesity and type 2 diabetes. The activation of c-jun NH2-terminal kinase(JNK),which is a member of the mitogen-activated protein kinase family (MAPK), can exert effects on the insulin signaling pathway and has been proved to be involved in the low-grade inflammation in obesity. In the present study, 3T3-L1 preadipocytes were first induced to differentiate into mature adipocytes, followed by incubation in different levels of insulin or glucose with or without pretreatment by the specific JNK inhibitor SP600125. The mRNA expression of adiponectin and resistin in adipocytes were evaluated by quantitative fluorescence PCR, respectively.Methods:1. Confluent 3T3-L1 preadipocytes were treated with 0.5mM IMX, 2uM dexamethasone and 10ug/ml insulin together for 48h, followed by standard medium DMEM culture with 10ug/ml insulin for another 48h,...