A Study On CCK-1R And Its MRNA Expression In Human Gallbladders With Cholestorel Stones

Objective Cholecystokinin(CCK) acts on cholecystokinin A receptors(CCK-1R) that activate G protein to cause gallbladder muscle contraction.Human gallbladders with cholesterol stones(CS) exhibit impaired muscle contraction compared with those with pigment stones(PS).CCK-lR dysfunction in gallbladder muscle membranes plays an important role in the formation of cholesterol stone.But the mechanisms are not fully understood.This study was designed to investigate the difference of the functions of the CCK-1R of gallbladder muscle membranes and the change in the expression levels of gallbladder CCK-1R messenger ribonucleic acid (mRNA) in two groups to determine whether there are any alterations in the functions of the CCK-1R and its mRNA expression that may account for the impairment of muscle contractility in human gallbladders with cholesterol stones.Methods The human gallbladder tissue and gallbladder bile was obtained by cholecystectomy or laparoscopic cholecystectomy performed for gallstone diseases in the department of general surgery in the First Affiliated Hospital of AnHui Medical University and the Hospital of Armed Police in Anhui. None of the patients had a history or clinical picture of acute cholecystitis or taked the medicine which could affect the gallbladder motility. All the patients with hepatitis,diabetes mellitus or liver cirrhosis were excluded.The height ,weight and body mass index (BMI) of the patients were investigated and the liver-kidney function,blood sugar and blood fat were collected. Fourty-one gallbladders were divided into two groups: cholesterol stone (CS, cholesterol composition in the gallstone ≥ 70%) and pigment stone(PS, cholesterol composition in the gallstone≤ 30%) according to the gallstones gross appearance and...