Analysis Of The Active Component In QC For Protecting Renal From Ischemia-reperfusion Injury

Renal ischemia-reperfusion injury(IRI) is one of the most common causes of acute renal failure(ARF) and transplanted kidney dysfunction. In spite of obvious progress in therapeutic methods, the mortality associated with ARF has not decreased substantially over the last 50 years. After primary selection, we found QC which has the protective function for IRI. Objective: We designed a series of experiments to find out the active component of QC for IRI to apply to clinic and to stabilitate the optimum extracting procedure. Methods: 1. QC is extracted in sequence by 95% ethanol, 60% ethanol and water. Water-soluble products are separated by ethanol sedimentation. 2. Male SD rats were randomly divided into 4 groups( 6 rats per group), which were respectively administered with salt water, F₁, F₂ and F3 for 3 days. In the 4^th day IRI rat modle was established by clamping left renal pedicel for 45min and removing the right kidney. After reperfusion for 24hr the serum Bun, Scr were detected. 3. Male SD rats were randomly divided into 3 groups( 6 rats per group), which were respectively administered with salt water, S₁ and S3 for 3 days. In the 4^th day IRI rat modle was established by clamping left renal pedicel for 45min and removing the right kidney. After reperfusion for 24hr the serum Bun, Scr were detected and the renal pathologic changes were observed. 4. Polysaccharides in S₁ were determined with the phenol-sulfuric acid method. Coomassie Brilliant Blue method was used to determined protein in S₁. 5. The optimum extracting procedure was selected by orthogonal test design. Result: 1. The levels of serum Bun, Scr and the renal pathologic changes were significantly decreased in group with S₁ treatment as compared with those in IRI group. 2. Polysaccharides content in S₁ was 55.33%, protein was 1.02%. 3. The optimum extracting procedure was determined with 10 times of water decocting 1.5 h and 3 times, and the solution were concetrated to lg/ml. Conclusion: Active component of QC for IRI was S₁, which basis was polysaccharides. The optimum extracting procedure was...