Use Of Quantitative Real-Time PCR To Study The Kinetics Of Extracellular DNA Released From Candida Albicans

Invasive candidiasis remains one of the leading causes of morbidity and mortality in immunocompromised and critically ill patients, with Candida albicans infections comprising 45% of all Candida bloodstream infections. Conventional blood cultures are time-consuming and not very effective in detecting yeast growth, although great progress has been made in recent years. Alternative procedures such as the detection of circulating antigens(or antibodies), enzymes or metabolic products lack sensitivity and specificity. Hence the requirements for strict diagnostic approaches become a very urgent issue in clinical mycological laboratory.In recent years, molecular biological techniques (especially PCR techniques) play an important role in the inchoate diagnostic of fungal infections. Real Time PCR is currently one of the promising methods to detect the pathogen of fungal infections in clinical samples.Currently there is controversial on which blood fraction is best suited for detection of invasive candidiasis. The objective of our study is to investigate the kinetics of DNA released from Candida albicans in vitro. We tried to use real-time PCR methods to detect the DNA of fungal pathogens, so as to establish the methods to detect invasive fungal pathogens earlier.