The Expetimental Study Of Differnent Rat Severed Limb Preservation Methods And The Effects To Blood Vessel And Skeletal Muscle

Objective: The severed tissues bring about a cascade of pathological changes due to damages of ischemia and hypoxia, leading to progressive degeneration of cells. When they step into the irreversible degenerative stage, the tissues inevitably come into necrosis. Therefore, it is of crucial importance for us to study and research how to preserve severed limbs to postpone metabolic process and relieve the ischemic damage of the tissues as possible as we can, so as to increase reimplant achievement ratio of severed limbs. Dryness and low temperature preservation is commonly used to protect tissue activity. Preservative fluid for severed tissues has been of wide used in spare-part surgery because of satisfactory effects, and ultra low temperature preservation has been applied to some clinical departments. In our present study, the model was established in rats which were subjected to severing limbs to investigate different effects on severed limbs of three methods: dryness and low temperature preservation, HC-A preservative fluid and ultra low temperature in liquid nitrogen. Preservative effects of three different methods were reflected by pathological and ultramicrostructural changes of vascular endothelium or media, and Na~+-K~+-ATPase activity of skeletal muscle at 8h, 24h and 72h respectively.Methods: Adult Wistar rats were used in the experiments, both male and female. Animals were randomly divided into 3 groups: normal dryness group, HC-A preservative fluid group, and ultra low temperature group, 15 rats in each group. Rats were anesthetized by ketamine. The hind limbs were cut at 1/3 forepart in the leg, and both of the right and left were obtained, preserved by three methods described as above.