The Empirical Study Of The Animal Model Of The RatBone Marrow Stem Cells's Culture, Introduced Into Muscle Skeletal Cells For Treatment Muscle Skeletal Injury

[Objective] To verificate rat mesenchymal stem cells(MSCs) in vitro culture ways and bionomics, to research the MSCs's multiple differentiated potentiality and their induced into muscle skeletal cells in vitro, to design a lot of animal models and injected the MSCs, the induced MSCs, the positive control cells into the animal models, to research MSCs differentiated condition and their morphologic changes, accordingly, to find the best restorative procedure of the muscle skeletal defection, expand the wide prospect for clinical therapy.[Methods] To culture the rat MSCs, the rat muscle skeletal cells(the chief use for the positive control), induced the tert-cells by 5-Aza, MyoD, TGF- β1, and IGF-1, to identify the induced MSCs in immunohistochemistry. Make animal models: 16 Wistar rats, 10 weeks old, weight 200g also, inject left leg the no water alcohol 0.2ml into the middle of the anterior tibial muscle and make it necrosis; inject right leg the stroke-physiological saline(SPSS) into the middle of the anterior tibial muscle. To random introduced four groups , every group has four rats, the first group is the negative control, only inject the medium; the second is the experiment group, inject the MSCs; the third is experiment group, inject the induced MSCs; the last group is the positive control, inject the muscle skeletal cells. To sign the every type cells by BrdU and collect them to wait for injection. 4h after animal modeling, begin to inject the cells into it. The injection site is the middle of the anterior tibial muscle. To draw the material from the models and observe the morphous changes and carry out the immunologic test in the third, the sixth, the ninth, the twelfth day.