Animal Experimental Study On Mucosal Immunization Of Sperm-Specific Lactate Dehydrogenase-Based DNA Vaccine

Sperm-specific lactate dehydrogenase, generally consisting of four copies of subunit C, is also referred to as lactate dehydrogenase C4, or LDH-C4. Specifically existing in mature testis tissue of mammals and being a key enzyme for sperm energy metabolism, it is closely related to spermatogenesis, spermatic metabolism and sperm capacitation. LDH-C4 has a very high immunogenicity. As is illustrated in animal experiments, LDH-C4 can induce the production of antibody to LDH-C4 in mammals. This very antibody has proved to cause a significant decrease in the fertility of animals, and this action is reversible. As the reproductive tract is rich in mucosal tissue, contraception can be achieved by mucosal immunization of LDH-C4-based DNA vaccine by taking advantage of the specificity and high immunogenicity of LDH-C4.In this study, the coding sequence of mLDH-C4 was amplified by RT-PCR, and the PCR product was inserted into pVAX1 to construct the recombinant eukaryotic expression vector pVAX1-mLDHC, and its expression effect was tested by transfecting HeLa cells and by using T7 Quick coupled transcription / translation system. BALB/c mice were immunized with pVAX1-hLDHC or pVAX1-hLDHC via mucosa. Sera and vaginal secretions were collected from mice of various groups and analyzed by Western blot and...