The Effect Of Liver-Derived B220～+CD11c～- Lymphoid Dendritic Cells On Metastatic Colon Carcinoma Of Liver In Vivo

Objective: To study the effect of liver-derived B220+CD11c- lymphoid dendritic cells expanded by plasmid-IL-3 and CD40L genes on metastatic colon carcinoma of liver in vivo.Methods: Liver-derived B220+CD11c- lymphoid dendritic cells were expanded by rapid tail-vein injection of plasmid-carring genes encoding IL-3 and CD40L in large volumes in Balb/c mice. Liver metastasis model of colon carcinoma was established by intrasplenic inoculation of colon adenocarcinoma cells (CT26) in Balb/c mice. Compared to control group respectively, the number and size of metastatic carcinoma nodules on liver surface were observed and counted. The splenic implanted carcinoma size and weight, the serum level of carcinoembryonic antigen (CEA) and the survival time of mice were detected .Results: 1. Liver nonparenchymal cells dramatically increased and located in the liver lobules in the treated group , but not in the control group. It is same as our prior results. It showed that liver-derived B220+CD11c- lymphoid dendritic cells were expanded. The mice were...