Anti-Hbv Activity Of Regulated Expression TNF-α

Objective: To construct tetracycline-dependent regulatory system in one vector, which contains two multiple cloning sites (MCS) for the convenient cloning of two target genes.Methods: The tetracycline(Tet)-dependent regulatory fragment (TO) (between NdeI and SacII sites)was amplified from pcDNA4TM/TO vector with PCR,which was inserted into pUC118 vector and sequenced.The counterpart fragment in pVITRO3 was replaced with correct Tet-dependent regulatory fragment, namely pVITRO3-TO vector. The Tet repressor gene (TR) encoding the Tet repressor from pcDNA6/TR vector was incorporating into the backbone of the pVITRO3-TO.The TNF-αgene was incorporating into the other backbone of the pVITRO3-TO-TR.Result: The tetracycline(Tet)-dependent regulatory fragment,the Tet repressor gene and TNF-αwere amplified successfully with PCR from its...