Development Of An Anti-infection Nano-HA Drug Delivery Microsphere And A Plastic Implant And Study On Its Releasing Character In Vitro

In this paper, we fabricated an anti-infection nano-hydroxypatite(nano-HA) drug delivery microsphere, providing a new local drug delivery system (DDS) for treating osteomyelitis. The nano-HA is used as the core carrier to load gentamicin (GM), then coating it with poly hydroxybutyrate-co-hydroxyvalerate/ polyethylene glycol(PHBV/PEG) to prepare the nano-HA - PHBV/PEG - GM microsphere. The characterization and in vitro release properties of the system were investigated. In order to develop a plastic implant, we mixing the microspheres with fibrin gelatin(FG) , then study on its antibacterial test and releasing character in vitro and observe its plasticity in the rabbits tibia.This experiment divides into three parts.Firstly, development of an anti-infection nano-HA drug delivery microsphere and study on its characterization.The nano-HA is used as the core carrier to load GM, then coating it with PHBV/PEG to prepare the nano-HA —PHBV/PEG —GM microsphere. The X-ray diffraction analysis performed phase analysis of HA nanoparticles. FT-IR spectra of composite microspheres and original materials were recorded using an FT-IR spectrophotometer. Microsphere size and morphology were obtained by transmission electron microscope.Result: The products of hydroxyapatite nanopaticles and nano-HA-GM and nano-HA—PHBV/PEG —GM microsphere are made well. The X-ray diffraction...