Study Of Anticancer Constituents Of Marine Fungus 96F197

In searching for bioactive fungal metabolites from ocean, 14 marine fungi exhibiting potent cytotoxic activity were discovered by MTT method using HepG-2 cell line from the marine fungi collected from Fillipine coast region. Further research revealed that their cytotoxic activity was all exclusively from the acetone extracts of mycelia instead of fermentation culture.A strain (No. 96F197) exhibiting potent cytotoxic activity was fermented in a large scale, and the acetone extract of the mycelia was studied by bioassay-guided isolation. The inhibitory activities on HePG-2 cell line proliferation of the fractions from the acetone extract of the mycelia were assayed by MTT method. Totally 15 compounds were purified from the active and inactive fractions by applying various chromatography techniques. The structures of 10 compounds including one peptide (2), five alkaloids (3, 8, 9, 12, 14 ), two polyhydroxysterols (10, 13), one amino acid (11), and mannitol (1) were determined through elucidation of MS and NMR spectral data together with chemical methods. SCI finder database search confirms that the peptide (2) and three alkaloids (3, 12, 14) are new compounds. Cytotoxic activity study showed the alkaloids (8, 9, 12, 14 ) and two polyhydroxysterols (10,13) exhibited inhibitory activity on the growth of HepG2 cell line.