Studies On The Antitumor Constituents Of Two Sponge-derived Fungi And The Approach To Explore Sponge-derived Microorganisms Through Genetic Engineering Technology

Sponge-derived microorganisms have become an important source for researches on antitumor drugs, because they can produce structurally novel and biologically active secondary metabolites. This work describes the study on the secondary metabolites of two sponge-derived antitumor active fungi, identified as Penicillium auratiogriseum (Sp-19) and unknown strain CTFS-15. The isolated compounds were evaluated for their antitumor activities. Besides, the work also describes the study on the approach to explore bioactive metabolites of sponge-derived uncultured symbionts through the genetic engineering technology.Two active fungi strains were fermented, respectively. The whole broth was extracted with ethyl acetate to give ferment extracts. Monitoring by the bioassay-guided isolation, the extracts were separated by means of modern chromatographic methods including silica gel column chromatography, Sephadex LH-20, RP-18 and reverse HPLC. Twenty seven compounds were isolated and twenty three of them were elucidated for structures by spectroscopic methods (UV,IR,MS,1D-NMR and 2D-NMR) and physicochemical properties. Compounds isolated from P. auratiogriseum are Auratiomide A(1), Auratiomide C(2), Anacine(3), Fructigenines A(4), Fructigenines B(5), Pseurotin A(6), Cyclopenin(7), Cyclopenol(8), 3-hydroxy-4 -(3-hydroxyphenyl)quinolin-2(1H)-one(9), O-Methylviridicatin(10), 3-methylquinazo lin-4(3H)-one(11), (2S,4S)-2,4,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one(12), (2S,4R)-2,4,8-trihydroxy-3,4-dihydronaphthalen-1(2H)-one(13), (3R,4R)-3,4,8-trihyd roxy-3,4-dihydronaphthalen-1(2H)-one(14), Ergosterol(15), N-(2-hydroxyphenyl)acet amide(16), N-(2-(methylamino)phenyl)acetamide(17) and Emodin(18). Compounds...