Effects Of Resuscitation After Selective Cerebral Ultra-deep Hypothermiac Blood Flow Occlusion On Expression Of C-fos And C-jun: An Experimental Studies On Monkey

Objective: To observe the expression of c-fos and c-jun in monkey of resuscitation after selective cerebral ultra-deep hypothermia and blood flow occlusion.Method: Ten 4-10 year-aged maca mulattas were divided into three groups: group A (four-vessel occlusion group), group B (identical temperature perfusion group) and group C (two-vessel occlusion group). Exposed bilateral exteral jugular veins (EJVs), bilateral exteral carotid artery (ECAs), bilateral internal carotid artery (ICAs) and bilateral internal jugular vein (IJVs), four-vessel occlusion group exposed bivertebral artery (VA). Right IJV intubated to monitoring central venous pressure (CVP). A catheter was inserted into right ICA to infuse cold Ringer's solution, two catheters were distally and proximally inserted into IJV to extract the hypo-thermic diluted blood for ultrafiltration and then perfuse the warm blood into the right venae cava inferior after rewarming. Total body was heparinizated before reduce the temperature. Both ICA were clamped 10 minutes (four-vessel occlusion group were clamped VA). Then the both EJVs and the left IJV were clamped about 60 minutes. 4 °C cold solution was immediately perfused into the right ICA to induce the brain cooling less 16℃. After 60 minutes Perfusion was stoped and blood vessels were recovered blood stream to cause brain physio-rewarming. Preoperative and postoperative examinated MRI. Operation changes of hemodynamics were measured; the dysfunctional scales of neurology were done every week.Immunohistochemical technique was used to examine the level of c-fos and c-jun protein expression in the four-vessel occlusion group, the identical temperature perfusion group and the two-vessel occlusion group, respectively.