Treatment Of Experimental Lung Metastasis In Mice By Intravenous Administration Of M Protein Gene Encapsulated In Cationic Liposome

Objective: Vesicular stomatitis virus (VSV) matrix (M) protein can directly induce apoptosis by inhibiting host gene expression. Our lab confirmed that the transfer of M protein gene can suppress malignant tumor growth in vitro and in vivo. The present study was designed to explore feasibility of intravenous delivery of M protein gene encapsulated in cationic liposome and observe the ability of suppress formation of experimental lung metastasis.Methods: Before animal tests DOTAP:Cholesterol-DNA complex was prepared. Animal tumor models were established by injecting CT26 tumor cell into BALB/c mice via tail vein. Then mice were randomly divided into 3 groups, and treated with Saline control(200ul), Lip-null(50ug DNA ,200ul) and Lip-MP (50ug DNA,200ul) via intravenous administration every two days for 6 times. The 5 mice per group were sacrificed 15 days after tumor implantation, lung weights were recorded, and the numbers of lung metastasis nodules of all groups were counted. Lung samples were evaluated by HE stain. Apoptosis of tumor cell in lung metastasis nodules was detected by TUNEL assays. The other mice were used to observe survival time. In...