An Experimental Study For Biocompatibility Of N-HA/PVA Hydrogel Keratoprosthesis

Part one: In vitro study of n-HA/PVA composite for keratoprosthesisObjective: Through cell culture in vitro to evaluate the cytotoxicity andhistocompatibility of n-HA/PVA (nano-hydroxyapatite/polyvinyl alcohol) hydrogel composite for keratoprosthesis.Methodes: There were four materials to be evaluated, big-porous and small-porous n-HA/PVA hydrogel composite, big-porous and small-porous PVA hydrogel. Every kind of materials was digested in F₁₂ nutrient culture for 24h to get the materials impregnant. Comeal fibroblasts were cultivated in materials impregnant and through methyl thiazolyl tetrazolium (MTT) colorimetry to assay the cytotoxicity. Corneal fibroblasts were also seeded onto the four materials in vitro. Three days later the growth of fibroblasts were observed by inverted microscope and scanning electron microscope(SEM).Results: The cytotoxicity scores of the materials with big-porous were both...