Cloning,Expression And Phylogenetic Research For The Hydrogenosomal Adenynate Kinase(AK) Of Trichomonas Vaginalis

Background and purpose: Trichomonas vaginalis (T.vaginalis ) is a flagellate protozoan that parasitizes the urogenital tract of humans and is the cause of trichomoniasis, which is a familiar sexually transmitted diseases . There are about 1,2000 crores of trichomoniasis sufferers annually in the world. In recent years, the sufferer quantity both of man and woman are going up, as a quarter of the quantity of the sexually transmitted disease sufferers. This disease is the mostly sexually transmitted diseases that threatened the healthy of human . Adenylate kinase(AK) is a important nucleotide phosphotransferases and play a central role in energy metabolism. For coping with energetic and synthetic challenges, T.vaginalis requires high activities of adenylate kinase .This enzyme are probably ubiquitous, presents in bacteria , archaebacteria and in various compartments of the eukaryotic cells, such as the cytosol, intermembrane space and matrix of mitochondria, and chloroplasts. It is also found in membrane-bounded organelles restricted to certain protest groups, such as hydrogenomes of trichomonads. The primary structure of nucleotide kinases from over twenty species has been deciphered .Based on the size of the proteins, this enzyme family can be subdivided into two subfamilies, larg and small nucleotide kinases. The large enzymes are over 24 kDa, while the small ones are around 21 kDa. This difference is due to the presence of a long stretch in the carboxy-terminal half of the large enzymes which is absent from the small ones. Information from vertebrates and yeasts reveals the presence of...