Function And Mechanism Of Clearing Lung Phlegm Reduction And Dredging The Channels And Collaterals In Bleomycin-induced Pulmonary Damage Of Rats

Object: In present study we repeated rat model of pulmonary fibrosis by using bleomycin and as same time treated with Clearing lung phlegm reduction and Dredging the channels and collaterals of Pro. Qiu Xinfan' s. Discusses the preventing and controlling function and the mechanism of this Fang in the model early time acute pulmonary alveolus inflammation and continues sends the lung fibrosis.Method: In this study,72 rats were divided into three groups: blank group, model group, treat group in random. On experimental day, the rats were intratracheally instilled with bleomycin (5mg/kg body weight)or sterile saline. On day3, 7,14,28 after instillation, 6 rats of each group were sacrificed and the lung tissues were harvested for manufacture the convention paraffin section: HE dyeing observation lung organization pathological change characteristic; MASSON dyeing observation lung organization fibrosis degree; The immunity histochemistry Sp determination TGF—β₁, MMP-2, iNOS in lung expression.Results:1. Lung organization inflammation response and fibrosis change process: Uses HE and the MASSON dyeing observation, in blank group the rat lung organization has not seen obviously exceptionally. The model group and the treat group rat 3rd, 7 days, the lung organization displays for the obvious acute inflammation response; The 14th day inflammation reduces, collagen deposition increase; 28th lung organizational structure destruction, The pulmonary alveolus cavity obviously reduces, Between the lung the nature is kept peace by the collagen filament the ciliary cell substitution, Forms the lung fibrosis the dynamic change process. Chinese native medicine group's...