The Alteration Of NF-κB, I-κBα, IL-6 Induced By Cigarette Smoke Extract And Effect Of Methylprednisolone In The Human Pulmonary Type Ⅱ

Objective: To observe the alteration of nuclear factor-κB (NF-κB), Inhibitor-κBa (I-κBα), interleukin-6 (IL-6) induced by cigarette smoke extract and effect of methylprednisolone in the human pulmonary type Ⅱ.Methods: A549 cells were cultured in vitro, and then divided into three groups: serum free DMEM, CSE-stimulated group; CSE plus methylprednisolone group, measured by inverted microscope, immunocytochemistry, Western-blot analysis and ELISA, respectively. Results: CSE could change the cells' morphology and activity, Western-blot analysis of total protein showed a rapid loss of I-κBα protein followed by its reappearance usually 60-90 min post CSE stimulation. Loss of I-κBα was preceded by a mobility shift, observed 5min continued turnover of I-κBα, NF-κB activation, IL-6 increase, methylprednisolone had an effect on the ability of CSE to cause I-KBa degradation, there was some obvious change in the time course of I-κBα protein resynthesis and NF-κB decreasion of activation, IL-6 increase, t here were significant between groups, (groups: F=19.633 P<0.01, times: F=14.88 P<0.01). Conclusion: methylprednisolone may suppress the activity of NF-kB by, increase the resynthesis of I-κBα, decrease cytokines IL-6, while weaken the cytotoxicity of CSE and may play a role in the anti-inflammatory treatment of respiratory diseases.