| Objective:Clinically, multidrug resistance mediated by P-gp is the major cause for the failure of cancer chemotherapy and the death of cancer patients. A urgent need still exists for the development of new RRA especially active monomer of natural products. One of the ways to reverse cancer multidrug resistance is to use chemicals that can directly block the drug-efflux function of P-glycoprotein. EGCG is one of the major components present in green tea. Experiments were carried out to investigate the effect of EGCG administrated with chemicals on experimental chemotherapy against xenografts derived from human MDR HCC cell line BEL-7404/Adr and Human oral epidermoid carcinoma KBV200 and the potential mechanism. In this study, we found that this naturally-occurring compound is able to down-regulating the membrane P-gp expression, and therefore, is a candidate agent with potential application in clinical cancer therapy.Methods:1.MTT was used to test the cytotoxicity of EGCG to splenic cells and HL-7721 cell lines.2.The models of BEL-7404/Adr xenograft in nude mice were established and received ADM and EGCG of desired drug and dosage for 20 days. In a BALB/C-nu/nu mouse model, cells of drug-sensitive KB and KBV200 cell lines were inoculated to yield tumors in opposite flanks. Nude mice with KBV200 xenograft was treated with VCR and EGCG of intraperitoneal injection for two weeks. Morphology of xenografts...
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