The Significance And Change Of The Amount Of Infiltrating Lymphocytes In The Liver Tissues Of Chronic Hepatitis B

Objective:To investigate the significance and change of the amount of T lymphocytes, B lymphocytes, natural killer cells and Kupffer cells in the liver tissues of chronic hepatitis B.Methods: By using immunohistochemical technology,CD3,CD57,CD20 and CD68 positive cells were detected in 58 liver-aspiration- biopsy-specimens from chronic HBV infection patients and in 20 normal liver- surgical- specimens from hepatic haemangioma patients. The positive staining cells were counted and analysed by pathological image instrument system.Results:(1)Histological observation: there was various degree of degeneration,necrosis and chronic lymphocytic inflammatory infiltration in the liver tissue. More serious infiltrated lymphocytes was observed in portal triads and the surrouding tissues.Besides these, more or less fibrotic tissues were observed in necrosis and portal triads.( 2 ) With the aggravating of liver inflammation , the content of alpha-fetoprotein(AFP)and a lanine transferases(ALT)increased(P<0.01),on the contrary ,albumin-globulin ratio decreased(P<0.01).The content of albumin(A)in grave hepatitis was higher than that in mild or moderate hepatitis(P<0.01).(3)Immunohistochemical observation: Most of T and B lymphocytes existed in portal area and fibrotic tissues; less B lymphocytes infiltrated in necrotic tissues; Majority of NK cells infiltrated in the areas of piecemeal necrosis,bridging necrosis and massing necrosis, less NK cells existed in fibrotic tissues and portal triads;Kupffer cells were mainly observed in the sinuses,fibrotic tissues and necrosis.