| Objective: Metastasis and recurrence have been become bottleneck to enhancing the survival rates of the HCC patients. Recently, some reports show that OPN may be a potential prognostic marker and a candidate target for cancer therapy. OPN might regulate the expression and active of VEGF, MMP-2 and uPA in some vitro studies. This study we investigated the expression of OPN in rat liver tissue and HCC tissue from the AFB1-induced rats hepatocar- cinogenesis and analysed the relationship between OPN and hepatocar- cinogenesis. At the same time, we investigated the expressions of OPN, VEGF, MMP-2, uPA and CD34 in the human HCC tissues from different developmental stages and analysed the roles of them in the prognosis, such as metastasis and recurrence.Methods: Detect the expressions of OPN mRNA and protein in the liver and HCC tissue from the AFB1- induced rats hepatocarcinogenesis by RT-PCR and immunohistochemistry. The paraffin-embedde human HCC tissues were divided into three groups: group 1 (36 cases), neither tumor metastasis nor recurrence within 18 months after HCC resection, group 2 (22 cases), with tumor metastasis, group 3 (22 cases ) , with recurrence within 18 moths after HCC resection. Detect the expressions of OPN, VEGF, MMP-2, uPA and CD34 in HCC tissues by immunohistochemistry.Results: The expressions of OPN mRNA and protein were increasd during the AFB1-induced rats hepatocarcinogenesis. The overall expression rates of OPN,VEGF,MMP-2 and uPA in human HCC tissue at the cytoplasm were 92.5%(74/80), 71.25%(57/80), 91.25%(73/80) and 80%(64/80)respectively. The expression of CD34 located at the endothelial cell of HCC tissue and the vascular endothelial cell in the strome . The expressions of OPN, VEGF,... |
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