The Translation And Quantitation Of The Metabolites Of Dipfluzine In Rat In Vivo

Dipfluzine (Dip), a novel piperazines calcium antagonist, selectively dilated vertebral artery, basilar artery and coronary artery. And the effects of Dip were more potent than those of flunarizine. In addition, Dip increased cerebral blood stream, improved brain ischemic injury, attenuated the brain infarct size induced by caremral ischemia, and inhibited platelet aggregation and thrombus formation. Dip may be potentially useful to treat ischemic brain injury, so after the research of pharmacokinetics characteristics of Dip, It is important to clarify the biotransformation of Dip in vivo and the quantitation of metabolites to guide the use of clinical drug aright. We studied the biotransformation of Dip in vivo and the quantitation of metabolites using HPLC in rat given drug by vein injection.Objective: To study the characteristics of transform and excretion of Dip in rats in vivo.Method: 1)Chromatogram condition: protection column: Diamonsil C18(5um,4.6mm×4mm); analytical column: Zorbax C8(5um,4.6mm×15mm). Mobile phase: 0.2% aminic acid as side A and methanol-acetonitrile-aminic acid (60:40:0.2) as side B. We use the manner of grads wash: the quantitation of B was from 0% to 90% within 50 min at flow rate of 1ml/min. The...