Role Of HO-1 In Secondary Brain Damage Following Intracerebral Hemorrhage And The Intervention Of Edaravone

Background and objectiveIntracerebral hemorrhage (ICH) is nontraumatic hemorrhage in brain parenchyma, which has dangerous sequeal,bad prognosis,high mutilation and fatality rate. The secondary brain edema and nerve injury are the important factors of unfavourable prognosis.The moieties of haematoma and their degradation products are the main cause of the secondary brain edema.Thrombase plays an important role in the early brain edema,while erythrocyte and its metabolic products may be the main cause of tardive edema. With the approach of experiments and researches, more and more attention are payed to the effect and mechanism of heme oxygenase,the rate-limiting enzyme of hemoglobin metabolism. Researches suggest that ICH induce the expression of HO-1 directly and indirectly.HO-1 mitigates oxidative injury when upregulating less than 5-fold.However, overexpression (more than 15-fold) of HO-1 can induce oxidative injury.Edaravone,one of the free radical scavengers,was used to treat acute cerebral infarction extensively.But it is not clearly whether it can be used to treat ICH patient.This research intend to explore the therapeutic efficacy and possible mechanism of edaravone in ICH treatment,and provide theoretical basement for ICH pathophysiologic mechanism.Method1. Animal preparation:A totle of 99 male Wistar rats were used in the present study.45 rats were used for immunohistochemical staining of HO-1.They were devided into three groups randomly:ICH group, Therapy group and Control group. Each group was devided into 1d,3d and 7d subgroups according to the phases.Each subgroup had 5 rats. The rest rats were used in brain edeme test, MDA detection and SOD energometry. They were also devided into three groups and three subgroups,but each subgroup had 6 rats.2. Animal model building: Used stereo-orientation technique making ICH model. The...