The Effect Of Antisense-human TIMP-1(hTIMP-1) TIMP-1 Transfection On The Expression Of And MMP-2 In The Lungs Of Rats With Pulmonary Fibrosis Induced By Bleomycin

Objective: Pulmonary fibrosis is a progressive pathological process, initiated by pulmonary parenchymal damage and perpetuated by inflammatory reaction. It is predominantly characterized by excessive accumulation of extracellular matrix (ECM) components in the lung. The process of pulmonary fibrosis formation is very complicated. The Reason of producing pulmonary fibrosis is different, but it is analogical about ECM metabolism abnormality. Excessive ECM deposition resulting from ECM degradation overwhelmed or ECM synthesis increased leads to pulmonary fibrosis. However, no effective treatment of pulmonary fibrosis is available clinically at present.Previous studies show that decreased degradation of ECM plays an important role in the process of excessive accumulation of ECM in recent years. Internal and foreign scholars all think that increasing the expression of tissue inhibitors of metalloproternases (TIMPs), in particular TIMP-1, suppresses the activity of matrix metalloproternases (MMPs), which contributes to the formation of pulmonary fibrosis by decreasing the degradation of ECM. Thus, it is also important to prevent fibrogenesis by suppressing the expression of TIMP-1. To study the roles of MMP-2 and TIMP-1 in pulmonary fibrosis, the protein and gene expressions of MMP-2 and TIMP-1 in rat pulmonary fibrosis models induced by bleomycin (BLM) were observed and recombinant retroviral vectors containing sense and antisense hTIMP-1 respectively were transfected into fibrotic lungs of different stages in vivo to investigate the changes of the endogenous TIMP-1 expression. The aim of this study is to observe the effectiveness of...