Construction Of Replication-deficient Recombinant Adenovirus Vector Expressing KAI1 And Investigation Of Its Anti-metastasis Mechanism In Pancreatic Cancer

KAI1/CD82, belongs to transmembrane 4 superfamily (TM4SF) and encodes a 29.6 KD transmembrane glycoprotein which has the ability to regulate the cell motility, differentiation and invasion, was originally identified as a suppressor of metaststic spread of tumor cells in a rat prostate model since 1995. Since then , many investigations have been focus on the links between KAI1 and tumor metastasis that decreased KAI1 mRNA expression correlated with the formation of metastasis in pancreatic cancer and also a direct correlation between loss of KAI1 expression and poor prognosis. Our previous studies also demonstrated that after transfected with KAI1 plasmid, the growth of pancreatic cancer cells slowed and the invasive ability attenuated in vitro; the number and volume of pancreatic cancer metastasis to lung and liver were greatly reduced in nude mice . Due to the limitation of plasmid transfection and its high transuding efficiency of adenovirus vector, we constructed a replication-deficient recombinant adenovirus vector expressing KAI1 to further investigate its anti-metastasis mechanism in pancreatic cancer and try to find out apotential means for cancer gene therapy.In this article, Ad-EasyTM system was used to construct Ad-KAI1 by recombination in E.coli. First, the 850bp KAI1 DNA...