Suicide Gene's Targeted Therapy In Laryngeal Carcinoma Controlled By HTERT Gene Promoter

AIM: To observe the killing effect of HSV-tk/GCV system on laryngeal carcinoma cells and the inhibitory rate in nude mice model under the control of hTERT(human telomerase reverse transcriptase) core promoter. It can seek a new way to resolve the problem of non-specific killing effect in gene therapy of laryngeal carcinoma.METHODS: (1) hTERT core promoter and tk gene were amplified by polymerase chain reaction and cloned into pCI-neo vector. Eukaryotic expression vectors containing HSV-tk gene under the control of hTERT core promoter and CMV promoter were constructed. (2) Eukaryotic expression vectors was transfected into Hep-2 cells and Ecv304 cells by liposome method. Transfected cells were then selected with G418 and reverse transcription polymerase chain reaction (RT-PCR) was used to detect the tk gene expression in Hep-2 cells and Ecv304 cells. After GCV was added 72...