| The gastric carcinoma (GC) is one of the most common malignant tumors in China, and its pathogenesis remains to be determined. Compared with the nuclear matrix proteins (NMPs) extracted from the normal gastric epithelial tissue and GC tissue, the GC NMPs differentially expressed in our previous work. It suggests that some NMPs might take part in the carcinogenesis and development of GC. However, which of NMPs expressed differentially in GC remains unknown. One critical anti-oncogene, p16 gene, is a novel gene in regulating cell cycle progression. The NMPs associated with it may play an important role in generation of tumors. Based on mentioned above, the aim of this study was to separate and identify the differentially expressed NMPs in both SGC-7901 and GES-1 cell lines by two-dimensional gel electrophoresis (2-DE), computer image analysis technique, matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS) and sequencing analysis, which may contribute to further study on pathogenesis of gastric carcinoma.The canceration is associated with cell cycle dys-regulation intimately. A typical cell cycle phases include four strict orders: G1→S→G2→M. It is the key process that the cell cycle CDKs are activated; while, the CDKs may be regulated up or down through corresponding cyclins and inhibitory proteins of CDKs. In eight kinds of the... |
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