Construction And Expression Of Recombinant Plasmid PET30a(+)-Ts43 Of 43kDa Antigen Gene Of Trichinella Spiralis

Trichinella spiralis is one of the most widespread parasites which can infect human being and more than 150 species of other mammals. Trichinellosis caused by Trichinella is a serious parasitic zoonosis. The mortality is from 3% to 30% if the patient with trichinellosis have not been diagnosed correctly and treated in time. Since human firstly detected Trichinella spiralis in 1835, the disease has not been controlled efficiently and diffused to the most parts of the world and regarded as re-emerging disease and has brought great loss to people in pig feeding and food industry. This disease is mainly caused by eating raw and semi-cooked pork, so it is important to detect Trichinella larvae in pork in order to prevent human trichinellosis. So, it is necessary to develop a quick and easy way to detect Trichinlla spiralis larvae in meat At present no specific rapid diagnostic techniques are available. With the develop of technology of molecular biology and its wide use in parasitology, gene recombinant antigen will be used for the quarantine of Trichinella larvae in meat and its products and immunodiagnosis of trichinellosis because recombinant antigen could be prepared in large quantity and have good antigen specificity. Modern bioengineering techniques have offered new approaches to study on this field. Equipped by these powerful techniques, researchers have expected to make some breakthrough by finding out more antigenic genes specific for Trichinella and producing recombinant antigens in large amount.In this study, we expect to clone and express the gene encoding for the 43kDa antigen of T. spiralis with the means of gene engineer. The genes encoding target protein amplified...