Biological Effect Of Radon On Target Cells Of Rats And Uranium Miners

Objective: To study biological effect of radon on target cells of rats and uranium miner and seek for the early biomakers of oxidative stress damage and DNA methylation.Methods: (1) Twenty male Wistar rats were randomly divided into four groups, one control group and three experimental groups exposed to radon. Ninety one male uranium miners from a Chinese uranium mine in Jiangxi province were also divided into four groups according to the cumulative exposure dose similar with the rats (>120WLM, 60~120WLM, 30~60WLM , 2~30WLM ). (2)The monitoring data of radon and its daughters from 1995 to 2004 were used to calculate annual and months average radon concentration. Level of radon was monitored by the method of solid nuclear traces.(3) Activity of SOD and content of MDA in peripheral blood were observed to compare differences among the groups. (4) The methylations of p16 and MGMT gene were determined by MSP,and associated with radon cumulative dose. (5) Association of GSTP1genotype with methylation of MGMT and p16 gene in sputum of uranium miners was studied.Results: (1) Radon concentration in May and September could be representative of the annual average concentration with an equilibrium factor of 0.36. (2)The confounding factors such as age, length of service, work hours per day, cigarette smoking and alcohol consumption, habitation history, and family history of lung cancer were no statistic difference in four groups.(3)The activity of SOD in human peripheral blood of each group was (116.5±12.3)U/ml, (89.6±8.3)U/ml, (63.6±8.9) U/ml and (40.9±7.6)U/ml, and the result in rats of each group was (1393.40±88.30)U/ml, (1198.60±132.44)U/ml, (1198.60±132.44)U/ml and (1098.60±50.90)U/ml, respectively. The content of MDA in human peripheral blood in each group was (1.1±0.4)nmol/ml, (2.1±0.3)nmol/ml, (3.3±0.5)nmol/ml and (5.3±0.9)nmol/ml, and the result in rats of each...