Exploration Of The Effectual Ingredient Of Schistosoma Japanese Egg Antigen On Experimental Autoimmune Encephalomyelitis

Objectives:1. To explore the establishment of EAE animal model by another encephalitogenic determinant of myelin proteolipid protein (residues 139-151) and its immunological characterization.2. To explore the immunomodulation potential of Schistosoma Japanese Egg Antigen for EAE and to find out the relationship between parasite infection and the onset of MS.3. To use Gel Filtration Chromatography to depart the Schistosoma Japanese Egg Antigen according to the molecular weight.4. To explore the effectual ingredient of the Schistosoma Japanese Egg Antigen with using each ingredient of Schistosoma Japanese Egg Antigen to treat EAE.Methods:1. Each SJL mouse was injected s.c. in the abdominal wall with PLP139-151, Mycobacteria H37RA and CFA, and Bordetella pertussis bacilli.2. EAE was induced by PLP139-151 in SJL mouse; all mice were weighted and examined daily for neurological signs.3. Try to depart the Schistosoma Japanese Egg Antigen with Gel Filtration Chromatography according to the molecular weight.4. The immunomodulation effect of each ingredient of Schistosoma Japanese Egg Antigen on EAE mice was studied by examination daily for neurological signs and pathology examination, to find out the effectual ingredient of the Schistosoma Japanese Egg Antigen.Results:1. Incidence of EAE in immunized female animal was 90%. Most mice received...