| Aim: It has been known that Rhubarb, a famous Chinese herb medicine, can delay the progression of chronic renal failure (CRF) through lessening tubulointerstitial fibrosis. However, the actions of Rhubarb on renal fibrosis are still mystery. In this study, we explore Rhein, the active component extracted from Rhubarb, on TGF-β -induced renal interstitial fibroblast activation.Methods: TGF-β ltreated normal rat renal interstitial fibroblast (NRK-49F) were cultured with or without Rhein at different concentrations. Cell number was counted, and cell cycle was analyzed by flow cytometry. The fibroblast activation was assessed by expression of α-smooth muscle actin (α-SMA), and deposition of Fibronectin (FN) by western blotting.Results: Rhein suppressed TGF-β1 induced fibroblast growth in time-course, and a dose-dependent manner, and blocked resting cells into S and G2/M phase. It was demonstrated that Rhein abolished a -SMA expression induced by TGF-β1 in fibroblast in a dose-dependent way. Meanwhile, we found that Rhein suppressed TGF-β1-induced FN deposition and its extracellular assembly in fibroblast.
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