The Neuroprotective Effects Of Short-time Postinsult Treatment With Lithium After Transient Focal Ischemia-reperfusion In Rats

Objective: By establishing SD rat models of transient focal ischemia-reperfusion, (1) To compare P-Akt protein levels in ischemia hippocampus after different doses of short-term lithium postinsult treatment,(2) To observe the effects on P-Akt in ischemia hippocampus and the neuroprotective effection with 3mmol/kg lithium postinsult treatment, (3) To investigate the mechanisms of neuroprotective action of lithium.Methods: Inserted with a nylon the right middle cerebral artery of rats was occluded(middle cerebral artery occlusion,MCAO) for 1.5h, then recirculation was instituted for various times before death.Experiment 1: SD rats were randomly divided into five subgroups: LI0(0mmol/kg), LI0.5(0.5mmol/kg), LIl(lmmol/kg), LI3(3mmol/kg) andLI5(0mmol/kg), and they were killed two days after ischemic insult.