| Background and Purpose: Female rodents producing endogenous estrogens are protected from stroke damage in comparison with male counterparts. This natural protection is lost after ovariectomy or reproductive senescence. Estrogen-related neuroprotection in association with animal models of cerebral ischemia has been documented in several recent reports.The aim of this study is to observe the neuroprotection of estrogens in male Sprague-Dawley rats with permanent cerebral ischemia and make further study to find out the dosage of E2 which can provided optimal neuroprotection.Methods: All the 80 heathly male Sprague-Dawley rats ,which were aged 3 months and weighed 200-230g were randomly divided into three groups:E2-pretreated group,in which the rats were given E2 by IP respectively in 10ug/kg weight,100ug/kg weight each day(7day) and 1mg/kg weight 1h before the operation, the animals plasma E2 were achieved low-physiological, physiological, super-physiologicall level accordingly. E2+TAM(tamoxifen)-pretreated group,in which the rats were given TAM in 3mg/kg weight each day(5 day) and E2 in 1mg/kg weight 1h before the operation. Control group in which the rats were given oil each day (7day).The model of cerebral ischemia were made by occluding middle cerebral artery using an intraluminal filament method.2 and 24 hours after focal cerebral ischemia, the neurological deficit scores and the death rate of rats were evaluated. 2,3,5-triphenyltetrazolium chloride (TTC) stain was used to assess the volume of infarction. Terminal transferase dUTP nick ending labeling (TUNEL) and immunohistochemistry were carried to observe neuron apoptosis and the expressions of bcl-2,bax,p53 in the penumbra region.
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