The Effect On Neural Behavior Of Offsprings Of KM Mice Primarily Infected Human Cytomegalovirus During Pregnancy

Objective: To explore the effect on neural behavior of offspring of KM mice primarily infected with Human Cytomegalovirus (HCMV) during different trimester. The aim is providing a scientific way to study the mechanism of learning disability (LD) and other neurobehavioral changes caused by HCMV congenital infection. Methods: HCMV (10^-3TCID50 in 0.5ml/mice) was separately given to 8-week-old fertilized female Kunming mice on 4, 10, 15 gestation days in experimental group with intra-abdominal injections. The equal quantity supernate of HF cell were inoculated in mice of control group at the same times. Each mouse was observed after inoculation. Brains of fetuses were collected by laparotomy before delivery, then observing pathology through light and electron microscope. Observe the outcome of pregnancy, postnatal mortality and complication. HCMV DNA in maternal and offspring's sera was tested. Neurobehavioral test such as Morris Water Maze (MWM), hole-board, Lashley III water maze, inclined plane, activities and stick climbing test were operated on offspring mice during 6-7 week-old. Results: Intrauterine infection can happen in three trimesters, and lead to growth retardation, fetal death and abnormity. The abnormality rates of fetus in group A₁, A₂, A₃,B₁, B₂, B₃ were 37.10%, 9.38%, 5.88%, 3.13%, 2.86% and 0%.The positive rate of HCMV DNA of offspring's sera in group A₁ A₂, A₃ were 58.33% (7/12) , 33.33%(4/12) and 16.67%(2/12), while in group B₁, B₂, B₃ were zero. Pathological damage such as swelling, necrosis, acidophil inclusion bodies in nucleus and viral granule was found in brain of infection groups under the microscopy. Compared with control group, the latency of the offspring mice in group A₁ was delayed (A₁: (30.21±12.74) s, A₁ vsB₁, A₂, A₃...