A Novel Peptide Binding To Cytoplasmic Domain Of Class A Scavenger Receptor Reduces Lipids Uptake By THP-1 Cells

Class A scavenger receptors (SR-A) are type II trimeric transmembrane glycoproteins mainly expressed on macrophages. A diverse group of polyanionic compounds have been identified as ligands for SR-A. The broad nature of ligand specificity in SR-A may explain receptor-multifaceted functions of macrophages such as adhesion, host defenses, apoptotic cell clearance, and atherogenesis. The cytoplasmic domain of the receptor is responsible for highly efficient internalization of the receptor-ligand complex, cells adhesion, and the receptor expression on cell surface. Our previous results have demonstrated that deletion of SR-A cytoplasmic domain has no effect on its binding to lipoprotein. However, expression of SR-A with truncated cytoplasmic domain in Chinese hamster ovary (CHO) cells did not cause lipid accumulation and foam cell formation, suggesting that the cytoplasmic domain of the receptor be an important target for therapeutic intervention of foam cell formation. In this study, we sought to identify peptides able...