The Research Of Molecular Mechanism During Human Cytomegalovirus Infection Causing Atherosclerosis

Part OneEffects of HCMV Infection on Expression of Adhession Molecules and RAGE of Human Vascular Endothelial CellsObjectiveTo study the mechanism of human cytomegalovirus infection that causes Atherosclerosis (AS) by investigating the sequential changes of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and the receptor for advanced glycation end products (RAGE) in human umbilical vein endothelial cells (HUVEC) with human cytomegalovirus infection (HCMV).MethodsHUVEC was cultured and then infected by HCMV. Expression of ICAM-1, VCAM-1 and RAGE mRNA from different infection stages were detected by reverse transcriptase polymerase chain reaction (RT-PCR).Results1. Effects of HCMV infection on the expression of ICAM-1 mRNA of HUVECICAM-1 mRNA was expressed at a low level at 0 hour, and began to increase at 4 hours after HCMV infection, reached the peak at 8 hours. After 12 hours, it declined obviously and remained at a relatively high level at 48 hours. There were enormous differences between all the other infection stages and 0 hour period (P<0.01).2 . Effects of HCMV infection on the expression of VCAM-1 mRNA of HUVEC...