Effect Of Resveratrol On Inflammation Gene Expression And CD40 Pathway Of Vascular Endothelial Cells

BACKGROUND: The cardioprotective effect of red wine owes to its polyphenols,especially resveratrol. And the anti-atherosclerosis effect of resveratrol derives fromits powerful anti-inflammatory effect. Atherosclerosis is an inflammatory processcharacteristic of endothelial dysfunction, so it is of significant importance toinvestigate the role of resveratrol in inflammatory endothelial cells. CD40 pathwayparticipates in the regulation of inflammatory reaction of atherosclerosis, so it is alsoessential to investigate whether resveratrol can protect cardiovascular system throughCD40 pathway.OBJECTIVE: In this study, the effect of resveratrol on inflammation geneexpression and CD40 pathway of HUVEC stimulated by TNF-a or sCD40L wasobserved.METHODS: Divide the cultivated HUVEC randomly into groups as the control,TNF-α(10μg/L), sCD40L(10μg/ml), resveratrol of different concertrations +TNF-αand resveratrol +sCD40L(10μg/ml). The empirical sequence of medication group isTNF-a or sCD40L followed by the preincubation of resveratrol. Observe the changeof cells in morphology with microscope and detect the expression of CD40 andE-selectin molecule on the surface of HUVEC with flow cytometric analysis, anddetect the transcriptional level of CD40, MMP-2, MMP-9, E-selectin, MCP-1 genewith RT-PCR.RESULTS: Observation of cell morphology reveals that after the stimulation ofTNF-a or sCD40L, HUVEC necrosis and defluxio, remnant cells present a swellingmorphous and array sparsenessly. After the preincubation of resveratrol, only a fewcells necrosis and defluxio and the remnant cells present a fairly morphous. Itindicates that resveratrol has a protective effect on the TNF-a or sCD40L injuredHUVEC.After the stimulation of TNF-a or sCD40L, the positive cell percentage and mean...