Experimental Research On Tissue-engineerring Artifical Bone Treating Bone Defect

Objective:To investigate the efficiency of treating bone defect by tissue-engineerring artificial bone based on rabbit bone marrow stromal cells and bioglass. Methods:Rabbit bone marrow stromal cell was separated for cell culture and subculture was done to increased the number of bone marrow stromal cell .A combined grafting material was made by bone marrow stromal cell and bioglass. The mixture,ilium and bioglass were used respectively to repair segmental defects created in the radius of rabbits. The repair capability for each of the treatment was assessed by figuration,radiography, histochemistry stains and scanning electron microscope,at 12 weeks after operation. Results:The quatity of new bone formation in the compound of bioglass and bone marrow stromal cells was more than other 3 groups. At the 12th week the defect in compound of the bone marrow stromal cells and bioglass is bridged with the appearance of marrow cavities. The bony healing could not been found in the blank controls,which were repaired by fibrous tissue after 12 weeks. The ability of new formation in compound of bone marrow stromal cells and bioglass was strongest.There was significant difference between the different groups. Conclusion:Bone marrow stromal cell is a kind of good bony stem cell ,which can be subdivided into bone cell . The mechanism of osteogenesis are both intramembranous and endochondral ossification . The tissue engineering in which the bone marrow stromal cell is used as seeding cell has a optimal perspective outlook.It is a satisfied method to repair bone defects.