Sciatic Nerve Injury Induced Changes In Behavior And Expression Of Growth Associated Protein In Dorsal Root Ganglion In Rats

objective: To observe the rats behavior change following sciatic nerve injury, to examine the effect of sciatic nerve injury on the expression of growth associated protein in the relevant dorsal root ganglion with the immunohistochemical technique, and to elucidate the relation between the expression of growth associated protein and the neuropahthic pain induced by sciatic nerve injury.Methods: Total of 250 Wistar rats were divided randomly into three groups: neurotomygroup (n=120) and neurotripsy group (n=120) and normal control group(n=10). According to the survival periods, the rats in the neurotomy group and neurotripsy group were divided into 3,7,14, 21,30 and 60-day subgroups with 20 rats in each subgroup.The bahavior changes were observed with autotomy score method following sciatic nerve injury, and the rats were sacrified at each time point respectively. Ipsilateral L5 dorsal root ganglions were removed, and immunohistochemical technique was used to detect the expression of growth associated protein.Results: All the 250 rats were involved in the analysis of result..(1) Autotomy score: After nerve injury, the incident rates of autotomy in the neurotomy subgroups were higher than those in the neurotripsy subgroups, and the severities were also worse. The percentage of autotomy score that more than 1 was 0.8 in 60-day subgroup of neurotomy group; more than 2 was 0.4. Otherwise, the percentage of autotomy score that more...