Study Of Separation And Antimicrobial Activity Of Extracts From Opuntia

In this paper ,extracts from opuntia was tested for its antimicrobial activities, including investigation of its antimicrobial spectrum ,MIC value,The feasible solvent with strongest activities was selected after comparing the differences of antimicrobial activities of different extracts from opuntia with different solvents by measuring their antimicrobial activities. The processing conditions of leaching active substances from opuntia were optimized. The sensitivity to universal factors of the extracts was studied and two active substances were extracted form opuntia and purified by silica gel column and SephadexG-75 gel chromatography. Eventually, it was invstigated that the metabolic mechanism of the experimented microbes affected by extracts from opuntia.1 .The extraction of antimicrobial compound from opuntia and the antimicrobial activities related to temperature, ultraviolet radiation, pH and their mutual influence to the extracts was investigated. The result showed that the extracts from opuntia had obvious antimicrobial activities especially to Escherichia coli and Staphylococcus aureus with the minimal inhibition concentration (MIC) : 6.25%.The middle is several mildews, the most weak is Saccharomyces cerevisiae.The extracts from opuntia was stable to temperature, ultraviolet radiation, antimicrobial activities were better when PH≤7.2.The antimicrobial activities of extraction by different solvents was quite different,methanol and 70%ethanol gave the best result followed by ethyl acetate > normal hexane. In the same action time, the higher the concentration of extraction ,the higher of the inhibition rate is. With the same concentration of extraction, the longer the action time will...